Journal: Vox Sanguinis

Article Title: Characterization of the in vitro quality parameters of cold‐stored platelets containing aggregates

doi: 10.1111/vox.70064

Figure Lengend Snippet: Platelet functional parameters are affected by aggregate formation. Apheresis platelets were transferred to cold storage within 8 h of collection, and components that developed aggregates during storage were compared to non‐aggregated controls at day 21. (a) PAC‐1 median fluorescence intensity was measured by flow cytometry, and the activation potential of GPIIb/IIIa was calculated as a ratio of stimulated (20 μM adenosine diphosphate [ADP] for 5 min at 37°C) and unstimulated fluorescence. Platelets were diluted in fresh plasma, and (b) spontaneous, (c) ADP‐ (20 μM) or (d) collagen‐induced (10 μg/mL) aggregation was measured by light transmission aggregometry. Platelets were diluted in fresh plasma, and thromboelastography (TEG) was used to assess the (e) R‐time and (f) maximum amplitude (MA) of the clot initiated by activation with kaolin and calcium chloride. Data represent individual data points, with mean (solid line) ± SD (error bars). Statistical comparisons were performed using a two‐tailed, unpaired t ‐test. Asterisk (*) indicates p < 0.05; ns indicates p > 0.05.

Article Snippet: The platelet clotting potential was measured by thromboelastography (TEG 5000, Haemoscope Corporation, Niles, IL, USA) using platelets diluted in plasma to 200 × 10 9 /L, re‐calcified and activated with kaolin, as previously described [ ].

Techniques: Functional Assay, Fluorescence, Flow Cytometry, Activation Assay, Clinical Proteomics, Transmission Assay, Two Tailed Test

Journal: Vox Sanguinis

Article Title: Characterization of the in vitro quality parameters of cold‐stored platelets containing aggregates

doi: 10.1111/vox.70064

Figure Lengend Snippet: Effect of filtration through a transfusion administration set on aggregates and other cold‐induced changes. Apheresis platelets were transferred to cold storage within 8 h of collection. Components that developed aggregates during storage were tested at day 21 and then gravity‐fed through an administration set containing a 200‐μm filter (filtered). (a) CD61+ events with a higher forward scatter than platelets (microaggregates) were measured by flow cytometry and reported per 10,000 platelets. (b) Platelet concentration and (c) mean platelet volume (MPV) were measured using a haematology analyser. (d) Extracellular vesicles staining positive for CD61 and annexin‐V were enumerated by flow cytometry. (e) PAC‐1 median fluorescence intensity was measured by flow cytometry. Platelets were diluted in fresh plasma and (f) spontaneous aggregation was measured by light transmission aggregometry. Thromboelastography (TEG) was used to assess the (g) R‐time and (h) maximum amplitude (MA) of the clot initiated by activation with kaolin and calcium chloride. Data represent individual data points. Statistical comparisons were performed using a two‐tailed, paired t ‐test. Asterisk (*) indicates p < 0.05; ns indicates p > 0.05.

Article Snippet: The platelet clotting potential was measured by thromboelastography (TEG 5000, Haemoscope Corporation, Niles, IL, USA) using platelets diluted in plasma to 200 × 10 9 /L, re‐calcified and activated with kaolin, as previously described [ ].

Techniques: Filtration, Flow Cytometry, Concentration Assay, Staining, Fluorescence, Clinical Proteomics, Transmission Assay, Activation Assay, Two Tailed Test